Filter: AllWBIHCIF-PIF/ICCAffinity

WB analysis using 80543-1-RR (same clone as 80543-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80543-1-RR (same clone as 80543-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80543-1-RR
Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

Connexin 43 Recombinant monoclonal antibody, PBS Only

Connexin 43 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 80543-1-PBS

Clone No.3O6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Connexin-43, GJA1, 3O6, CX43, Gap junction alpha 1 protein

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

80543-1-PBS targets Connexin 43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag25533 Product name: Recombinant human Connexin 43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 232-382 aa of BC026329 Sequence: FFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI Predict reactive species
Full Name	gap junction protein, alpha 1, 43kDa
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	43 kDa
GenBank Accession Number	BC026329
Gene Symbol	Connexin-43
Gene ID (NCBI)	2697
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17302
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).