Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, pig brain tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80543-1-RR targets Connexin 43 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag25533 Product name: Recombinant human Connexin 43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 232-382 aa of BC026329 Sequence: FFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI Predict reactive species |
| Full Name | gap junction protein, alpha 1, 43kDa |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC026329 |
| Gene Symbol | Connexin-43 |
| Gene ID (NCBI) | 2697 |
| RRID | AB_2935545 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17302 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| IHC protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| WB protocol for Connexin 43 antibody 80543-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Connexin 43 Recombinant monoclonal antibody (3O6), catalog number 80543-1-RR, has 2 citations. They appear in Advanced Materials (IF 29.1), covering myocardial infarction repair via conductive hydrogel patches in rat tissue (IF application), and Frontiers in Bioscience (Landmark Ed) (IF 4.1), addressing conditional immortalization of human cardiac fibroblasts for pro-fibrotic and anti-fibrotic drug screening (IF application). Associated research fields include cardiac tissue engineering and fibrosis biology.
| Species | Application | Title |
|---|---|---|
Adv Mater Injectable Conductive Hydrogel Patch with Spatiotemporally Tailored Asymmetric Adhesion for Myocardial Infarction Repair. | ||
Front Biosci (Landmark Ed) Conditional Immortalization of Human Cardiac Fibroblasts for Pro-Fibrotic and Anti-Fibrotic Drug Screening. |
Reviews
What customers say
One user reviewed this antibody for immunofluorescence on mouse brain slices at a 1:2000 dilution, giving it a 5-star rating. They reported very nice staining with signal contained in the expected locations, indicating good specificity and performance for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sama (Verified Customer) (03-26-2026) | Very nice staining - contained where it is expected
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