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Cryptochrome 1 Polyclonal antibody, PBS Only (13474-1-PBS)

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 13474-1-AP (same clone as 13474-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 13474-1-AP (same clone as 13474-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13474-1-AP (same clone as 13474-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 13474-1-AP (same clone as 13474-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 13474-1-AP (same clone as 13474-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.
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1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Cryptochrome 1 Polyclonal antibody, PBS Only

Name: Cryptochrome 1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13474-1-PBS

Cryptochrome 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 13474-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CRY1, CRY 1, Cryptochrome-1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13474-1-PBS targets Cryptochrome 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4270 Product name: Recombinant human CRY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 236-586 aa of BC030519 Sequence: RPRMNANSLLASPTGLSPYLRFGCLSCRLFYFKLTDLYKKVKKNSSPPLSLYGQLLWREFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLSCSSFFQQFFHCYCPVGFGRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNAPEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQLSRYRGLGLLASVPSNPNGNGGFMGYSAENIPGCSSSGSCSQGSGILHYAHGDSQQTHLLKQGRSSMGTGLSGGKRPSQEEDTQSIGPKVQRQSTN Predict reactive species
Full Name	cryptochrome 1 (photolyase-like)
Calculated Molecular Weight	586 aa, 66 kDa
Observed Molecular Weight	60-66 kDa
GenBank Accession Number	BC030519
Gene Symbol	CRY1
Gene ID (NCBI)	1407
RRID	AB_10697652
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16526
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.