Cyclin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 28603-1-AP

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

---

Validation Data Gallery

• 

  WB analysis using 28603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28603-1-AP (Cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28603-1-AP

  WB result of Cyclin B1 antibody (28603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.

• 

  IP experiment of HeLa using 28603-1-AP

  IP result of anti-Cyclin B1(IP:28603-1-AP, 4ug; Detection:28603-1-AP 1:2000) with HeLa cells lysate 1800 ug.

• 

  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 2 publications below

Product Information

28603-1-AP targets Cyclin B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin B1 fusion protein Ag29426
• Full Name: cyclin B1
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC006510
• Gene symbol: CCNB1
• Gene ID (NCBI): 891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes; Authors - Jian Chen
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human,rat	WB,IHC	Am J Physiol Lung Cell Mol Physiol; KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension; Authors - Xiaochen Li
human	WB	Front Immunol; Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma.; Authors - Yongbiao Huang
human	WB	J Inflamm Res; Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma.; Authors - Yiguan Qian
human	WB	Oncol Res; Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma; Authors - Huan Ma