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- KD/KO Validated
Cyclin B1 Polyclonal antibody
Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (4)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28603-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human skin cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 24 publications below |
IHC | See 2 publications below |
Product Information
28603-1-AP targets Cyclin B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cyclin B1 fusion protein Ag29426 |
Full Name | cyclin B1 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 55-60 kDa |
GenBank accession number | BC006510 |
Gene symbol | CCNB1 |
Gene ID (NCBI) | 891 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
Protocols
Product Specific Protocols | |
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WB protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IHC protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IF protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
IP protocol for Cyclin B1 antibody 28603-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Clin Transl Med Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes | ||
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
Am J Physiol Lung Cell Mol Physiol KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension | ||
Front Immunol Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma. | ||
J Inflamm Res Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma. | ||
Oncol Res Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma |