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Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (4)





Cat no : 28603-1-AP


CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

Tested Applications

Positive WB detected inHT-29 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HT-29 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28603-1-AP targets Cyclin B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin B1 fusion protein Ag29426
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006510
Gene symbol CCNB1
Gene ID (NCBI) 891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.


Product Specific Protocols
WB protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IHC protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IF protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IP protocol for Cyclin B1 antibody 28603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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