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Cyclin B1 Polyclonal antibody

Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28603-1-AP

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1



Tested Applications

Positive WB detected inHT-29 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28603-1-AP targets Cyclin B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin B1 fusion protein Ag29426
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC006510
Gene symbol CCNB1
Gene ID (NCBI) 891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IHC protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IF protocol for Cyclin B1 antibody 28603-1-APDownload protocol
IP protocol for Cyclin B1 antibody 28603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes

Authors - Jian Chen
humanWB

Cancer Lett

Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.

Authors - Xintian Chen
human,ratWB,IHC

Am J Physiol Lung Cell Mol Physiol

KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension

Authors - Xiaochen Li
humanWB

Front Immunol

Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma.

Authors - Yongbiao Huang
humanWB

J Inflamm Res

Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma.

Authors - Yiguan Qian
humanWB

Oncol Res

Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma

Authors - Huan Ma