Validation Data Gallery
|Positive WB detected in||HT-29 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human skin cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HT-29 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
28603-1-AP targets Cyclin B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin B1 fusion protein Ag29426|
|Full Name||cyclin B1|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC006510|
|Gene ID (NCBI)||891|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
|Product Specific Protocols|
|WB protocol for Cyclin B1 antibody 28603-1-AP||Download protocol|
|IHC protocol for Cyclin B1 antibody 28603-1-AP||Download protocol|
|IF protocol for Cyclin B1 antibody 28603-1-AP||Download protocol|
|IP protocol for Cyclin B1 antibody 28603-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Clin Transl Med
Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes
Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.
Am J Physiol Lung Cell Mol Physiol
KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension
Exploring the Prognostic Value, Immune Implication and Biological Function of H2AFY Gene in Hepatocellular Carcinoma.
J Inflamm Res
Pan-Cancer Transcriptomic Analysis Identifies PLK1 Crucial for the Tumorigenesis of Clear Cell Renal Cell Carcinoma.
Therapeutic Targeting PLK1 by ON-01910.Na Is Effective in Local Treatment of Retinoblastoma