CoraLite® Plus 488-conjugated Cyclin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin B1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28603

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

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Validation Data Gallery

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  IF Staining of HT-29 using CL488-28603

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

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  IF Staining of HeLa using CL488-28603

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Cyclin B1 antibody (CL488-28603) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HT-29 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28603 targets Cyclin B1 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin B1 fusion protein Ag29426
• Full Name: cyclin B1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC006510
• Gene Symbol: CCNB1
• Gene ID (NCBI): 891
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.