CoraLite®594-conjugated Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C5G11

Cat no : CL594-67065

Synonyms

CAK, CCNH, cyclin H, MO15 associated protein, p34, p37

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Validation Data Gallery

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  IF Staining of PC-3 using CL594-67065

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite®594 Cyclin H antibody (CL594-67065, Clone: 2C5G11 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of PC-3 using CL594-67065

  1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Cyclin H (CL594-67065, Clone:2C5G11) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: PC-3 cells
• Positive FC detected in: PC-3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67065 targets Cyclin H in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin H fusion protein Ag28556
• Full Name: cyclin H
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005280
• Gene Symbol: CCNH
• Gene ID (NCBI): 902
• RRID: AB_2920082
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.