Validation Data Gallery
|Positive WB detected in||Colo320 cells, COLO 320 cells|
|Positive IHC detected in||human colon tissue, human appendicitis tissue, human urothelial carcinoma tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HT-29 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:51200|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
60183-1-Ig targets Cytokeratin 20 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Cytokeratin 20 fusion protein Ag11336|
|Full Name||keratin 20|
|Calculated molecular weight||424 aa, 48 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC031559|
|Gene ID (NCBI)||54474|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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