Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human and More (1)







Cat no : 60183-1-Ig


Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT

Tested Applications

Positive WB detected inColo320 cells, COLO 320 cells
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human urothelial carcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human colon tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:51200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60183-1-Ig targets Cytokeratin 20 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11336
Full Name keratin 20
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC031559
Gene Symbol KRT20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.


Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
IHC protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
IF protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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