Validation Data Gallery
|Positive WB detected in
|Colo320 cells, COLO 320 cells
|Positive IHC detected in
|human colon tissue, human appendicitis tissue, human urothelial carcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HT-29 cells, human colon tissue
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:1000-1:51200
|IF : 1:400-1:1600
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60183-1-Ig targets Cytokeratin 20 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2b
|Cytokeratin 20 fusion protein Ag11336
|Calculated molecular weight
|424 aa, 48 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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Construction of artificial human peritoneal tissue by cell-accumulation technique and its application for visualizing morphological dynamics of cancer peritoneal metastasis.