Cytokeratin 20 Monoclonal antibody

Cytokeratin 20 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4D10A4

Cat no : 60183-1-Ig

Synonyms

Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT



Tested Applications

Positive WB detected inColo320 cells, COLO 320 cells
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human urothelial carcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:51200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 3 publications below

Product Information

60183-1-Ig targets Cytokeratin 20 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 20 fusion protein Ag11336
Full Name keratin 20
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031559
Gene symbol KRT20
Gene ID (NCBI) 54474
RRIDAB_10858399
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
IHC protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
IF protocol for Cytokeratin 20 antibody 60183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Mol Gastroenterol Hepatol

Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer.

Authors - Yuli Wang
humanIF

Front Immunol

Fusobacterium nucleatum Facilitates M2 Macrophage Polarization and Colorectal Carcinoma Progression by Activating TLR4/NF-κB/S100A9 Cascade.

Authors - Lijun Hu
humanIF

Biochem Biophys Res Commun

Construction of artificial human peritoneal tissue by cell-accumulation technique and its application for visualizing morphological dynamics of cancer peritoneal metastasis.

Authors - Yoshiya Asano