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Cytokeratin 4 Polyclonal antibody, PBS Only (16572-1-PBS)

Filter: AllWBIHCIF-PIF/ICC

WB analysis of A431 using 16572-1-AP (same clone as 16572-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
WB analysis of human bladder using 16572-1-AP (same clone as 16572-1-PBS)
human bladder tissue were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IF Staining of mouse skin using 16572-1-AP (same clone as 16572-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 16572-1-AP (same clone as 16572-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

Cytokeratin 4 Polyclonal antibody, PBS Only

Name: Cytokeratin 4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16572-1-PBS

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 16572-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16572-1-PBS targets Cytokeratin 4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9854 Product name: Recombinant human KRT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-534 aa of BC042174 Sequence: NLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR Predict reactive species
Full Name	keratin 4
Calculated Molecular Weight	534 aa, 57 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC042174
Gene Symbol	Cytokeratin 4
Gene ID (NCBI)	3851
RRID	AB_2134041
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19013
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.