CoraLite®594-conjugated DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3B6D2

Cat no : CL594-60082

Synonyms

DACH, DACH1, Dachshund homolog 1, FLJ10138



Tested Applications

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 60082-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-60082 targets DACH1 in IF applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DACH1 fusion protein Ag4474
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
GenBank accession numberBC021219
Gene symbol DACH1
Gene ID (NCBI) 1602
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110 kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 DACH1 antibody CL594-60082Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EBioMedicine

Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.

Authors - Xiang Hu