CoraLite® Plus 488-conjugated DBR1 Monoclonal antibody

• Featured Product
• KD/KO Validated

DBR1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B5A7

Cat no : CL488-66951

Synonyms

DBR1, Lariat debranching enzyme

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Validation Data Gallery

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  IF Staining of A431 using CL488-66951

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DBR1 antibody (CL488-66951, Clone: 3B5A7 ) at dilution of 1:100, CL594-Phalloidin (red).

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  IF Staining of A431 using CL488-66951

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DBR1 antibody (CL488-66951, Clone: 3B5A7 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66951 targets DBR1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DBR1 fusion protein Ag8830
• Full Name: debranching enzyme homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 544 aa, 62 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC009472
• Gene Symbol: DBR1
• Gene ID (NCBI): 51163
• RRID: AB_2919395
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.