DCP1A Recombinant antibody

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  WB analysis using 85047-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85047-5-RR (DCP1A antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85047-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (85047-5-RR, Clone: 242553F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DCP1A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85047-5-RR

Clone No.242553F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, SW480 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85047-5-RR targets DCP1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCP1A fusion protein Ag18027
• Full Name: DCP1 decapping enzyme homolog A (S. cerevisiae)
• Calculated Molecular Weight: 582 aa, 63 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC007439
• Gene Symbol: DCP1A
• Gene ID (NCBI): 55802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.