Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue, human testis tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
66010-1-Ig targets DDB1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||DDB1 fusion protein Ag17903|
|Full Name||damage-specific DNA binding protein 1, 127kDa|
|Calculated molecular weight||1140 aa, 127 kDa|
|Observed molecular weight||127 kDa|
|GenBank accession number||BC011686|
|Gene ID (NCBI)||1642|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.
Human herpesvirus 6A U4 inhibits proteasomal degradation of amyloid precursor protein.
Degradation of SAMHD1 by Vpx is Independent of Uncoating.
J Clin Invest
Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.