DDX23 Recombinant antibody

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  WB analysis using 85763-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85763-4-RR (DDX23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85763-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX23 antibody (85763-4-RR, Clone: 243147B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85763-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85763-4-RR against Human DDX23 were performed. The affinity constant is below 1 pM.

DDX23 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85763-4-RR

Clone No.243147B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

100 kDa U5 snRNP-specific protein, DEAD box protein 23, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX23, prp28

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HEK-293T cells, K-562 cells, A549 cells, NIH/3T3 cells, fetal human brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85763-4-RR targets DDX23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX23 fusion protein Ag0258
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96-100 kDa
• GenBank Accession Number: BC002366
• Gene Symbol: DDX23
• Gene ID (NCBI): 9416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BUQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.