DHODH Polyclonal antibody

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  WB analysis using 14877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 14877-1-AP

  IP result of anti-DHODH (IP:14877-1-AP, 4ug; Detection:14877-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 14877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DHODH antibody (14877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 14877-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human DHODH (14877-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

DHODH Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 14877-1-AP

Publications(93)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

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Tested Applications

• Positive WB detected in: A2780 cells, MCF-7 cells, SKOV-3 cells, mouse heart tissue, mouse ovary tissue, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 86 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

14877-1-AP targets DHODH in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6649

  Product name: Recombinant human DHODH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-395 aa of BC065245

  Sequence: MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

• Full Name: dihydroorotate dehydrogenase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC065245
• Gene Symbol: DHODH
• Gene ID (NCBI): 1723
• RRID: AB_2091723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02127
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.; Authors - Chao Mao ; KO Validated
human,mouse	WB	Science; Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.; Authors - Issam Ben-Sahra
human,mouse	WB	Cancer Discov; PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.; Authors - Deepti Mathur
mouse	IHC	Cancer Cell; Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma; Authors - William D Gwynne ; KO Validated
mouse	WB	Cell Metab; Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.; Authors - Christopher J Halbrook ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Prakash (Verified Customer) (10-17-2025)

working very good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: bladder cancer cell lines

FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded kidneys were stained for Dhodh (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

DHODH Antibody Immunofluorescence validation (1:200 dilution) in Mouse kidney (Cat no:14877-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Highly specific detection of both mouse and human DHODH.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human RCC4 cells (lane1), Mouse kidney (lane2)

DHODH Antibody Western Blot, validation (1:1000 dilution) in Human RCC4 cells (lane1), Mouse kidney (lane2) (Cat no:14877-1-AP)