mDia1 Polyclonal antibody

• Featured Product
• KD/KO Validated

mDia1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 20624-1-AP

Synonyms

DIAPH1, Diaphanous-related formin-1, Diaphanous related formin 1, DIAPH 1, DIAP1

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Validation Data Gallery

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  WB analysis using 20624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20624-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20624-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 20624-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20624-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20624-1-AP

  IP result of anti-mDia1 (IP:20624-1-AP, 4ug; Detection:20624-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse kidney using 20624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20624-1-AP (mDia1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20624-1-AP

  Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (20624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of Hela cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 20624-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mDia1 (20624-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, human skeletal muscle tissue, COS-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HUVEC cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• ChIP: See 1 publications below

Product Information

20624-1-AP targets mDia1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: mDia1 fusion protein Ag14523
• Full Name: diaphanous homolog 1 (Drosophila)
• Calculated Molecular Weight: 1272 aa, 141 kDa
• Observed Molecular Weight: 140-150 kDa, 70 kDa
• GenBank Accession Number: BC007411
• Gene Symbol: mDia1
• Gene ID (NCBI): 1729
• RRID: AB_10858618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Circ Res; Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.; Authors - Touré Fatouma F
mouse	WB	Dev Cell; Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.; Authors - Fernando R Valencia ; KD Validated
human	WB,IHC	J Cell Biol; Dia1 coordinates differentiation and cell sorting in a stratified epithelium.; Authors - Robert M Harmon
human	WB, IF	Mol Biol Cell; Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.; Authors - Jessica D Arden
human	WB	Cells; The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.; Authors - Kathryn N Becker
human	WB	PLoS One; A Novel Role for p115RhoGEF in Regulation of Epithelial Plasticity.; Authors - Swapnil S Kher ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Detects a band around 150kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

mDia1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20624-1-AP)