Tested Applications
| Positive WB detected in | HeLa cells, human heart tissue, human skeletal muscle tissue, COS-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HUVEC cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20624-1-AP targets mDia1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14523 Product name: Recombinant human DIAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1211-1272 aa of BC007411 Sequence: AFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS Predict reactive species |
| Full Name | diaphanous homolog 1 (Drosophila) |
| Calculated Molecular Weight | 1272 aa, 141 kDa |
| Observed Molecular Weight | 140-150 kDa, 70 kDa |
| GenBank Accession Number | BC007411 |
| Gene Symbol | mDia1 |
| Gene ID (NCBI) | 1729 |
| RRID | AB_10858618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60610 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IF protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IHC protocol for mDia1 antibody 20624-1-AP | Download protocol |
| IP protocol for mDia1 antibody 20624-1-AP | Download protocol |
| WB protocol for mDia1 antibody 20624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The mDia1 antibody (Cat# 20624-1-AP) has 24 citations published in journals including Circulation Research, Advanced Science, Developmental Cell, Cell Reports, Current Biology, Journal of Cell Biology, Molecular Biology of the Cell, PLoS ONE, mBio, Clinical Immunology, Cancers, Biophysical Journal, and Physiology & Biology. Research fields cover cytoskeletal dynamics, cell migration, vascular remodeling, glioblastoma/cancer invasion, EMT, wound healing, immune regulation, hematopoiesis, stroke, and infectious disease.
| Species | Application | Title |
|---|---|---|
Circ Res Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways. | ||
Adv Sci (Weinh) Cockroach-Derived Leucokinin VIII Peptide Accelerates Diabetic Skin Wound Healing by Enhancing Keratinocyte Filopodia Formation. | ||
Adv Sci (Weinh) Coupling of Perinuclear Actin Cap and Nuclear Mechanics in Regulating Flow-Induced Yap Spatiotemporal Nucleocytoplasmic Transport | ||
Int J Biol Sci Slc1a2-mediated Glutamate Transport Promotes Dendritic Cell Immunity through Sema3A-regulated Cytoskeletal Remodeling and Migration. | ||
Dev Cell Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.
| ||
Cell Rep Opposing roles of ZEB1 in the cytoplasm and nucleus control cytoskeletal assembly and YAP1 activity |
Reviews
What customers say
The single review for this product reports a 5-star rating for Western Blot use on HUVEC cells at a 1:1000 dilution. The user confirmed the antibody detects a band around 150 kDa, consistent with the expected target size. Overall, the feedback is positive, indicating reliable performance and specific detection in the tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (11-20-2023) | Detects a band around 150kDa.
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