DKC1 Polyclonal antibody, PBS Only

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• DKC1 Polyclonal antibody, PBS Only (25420-1-PBS)

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  WB analysis of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25420-1-AP (same clone as 25420-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 25420-1-AP (same clone as 25420-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 25420-1-AP (same clone as 25420-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 25420-1-AP (same clone as 25420-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25420-1-AP (same clone as 25420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25420-1-AP (same clone as 25420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DKC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25420-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

25420-1-PBS targets DKC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7992

  Product name: Recombinant human DKC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 157-514 aa of BC009928

  Sequence: VRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRFVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

• Full Name: dyskeratosis congenita 1, dyskerin
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC009928
• Gene Symbol: DKC1
• Gene ID (NCBI): 1736
• RRID: AB_2880071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60832
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.