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CoraLite®594-conjugated DLC1 Monoclonal antibody

DLC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G10H12

Cat no : CL594-66894

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A375 cells using CoraLite®594-conjugated DLC1 Monoclonal antibody (CL594-66894)

    IF Staining of A375 using CL594-66894

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using CoraLite®594 DLC1 antibody (CL594-66894, Clone: 1G10H12 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66894 targets DLC1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DLC1 fusion protein Ag24404
Full Name deleted in liver cancer 1
Calculated Molecular Weight 171 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.

Protocols

Product Specific Protocols
IF protocol for CL594 DLC1 antibody CL594-66894Download protocol
Standard Protocols
Click here to view our Standard Protocols