DLD Monoclonal antibody

DLD Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E7G5

Cat no : 67702-1-Ig

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3



Tested Applications

Positive WB detected inHuH-7 cells, NIH/3T3 cells, HSC-T6 cells, L02 cells, HepG2 cells, Hela cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67702-1-Ig targets DLD in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DLD fusion protein Ag9680
Full Name dihydrolipoamide dehydrogenase
Calculated molecular weight 509 aa, 54 kDa
GenBank accession numberBC018696
Gene symbol DLD
Gene ID (NCBI) 1738
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for DLD antibody 67702-1-IgDownload protocol
IHC protocol for DLD antibody 67702-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols