DLX6 Recombinant antibody

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  WB analysis using 85946-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85946-1-RR (DLX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 85946-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 85946-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 85946-1-RR (DLX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85946-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85946-1-RR against Human DLX6 were performed. The affinity constant is 5.65 nM.

DLX6 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85946-1-RR

Clone No.250408B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Homeobox protein DLX-6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85946-1-RR targets DLX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DLX6 fusion protein Ag19724
• Full Name: distal-less homeobox 6
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC069363
• Gene Symbol: DLX6
• Gene ID (NCBI): 1750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P56179
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Distal-less homeobox 6 (DLX6) has been reported to play important roles in the development of craniofacial structures, inner ear, limb, and brain. DLX6 was significantly highly expressed in oral cancer tissues in The Cancer Genome Atlas database. DLX6 promotes cell proliferation and suppresses cell apoptosis in oral cancer cells. EGFR-CCND1 pathway might be the potential mechanism participating in the regulating axis (PMID: 33215805).