Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24813-1-AP targets DMGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20529 Product name: Recombinant human DMGDH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-613 aa of BC022388 Sequence: ARESYGFNNIVGYPKEERFAGRPTQRVSGLYQRLESKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDSIRLLDHLFANVIPKVGFTNISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLRWIEEEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQTKSLKVSNIPVTAIRISYTGELGWELYHRREDSVALYDAIMNAGQEEGIDNFGTYAMNALRLEKAFRAWGLEMNCDTNPLEAGLEYFVKLNKDQNSCFAHFKEENGWVSRWAIRPY Predict reactive species |
| Full Name | dimethylglycine dehydrogenase |
| Calculated Molecular Weight | 866 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC022388 |
| Gene Symbol | DMGDH |
| Gene ID (NCBI) | 29958 |
| RRID | AB_2879739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DMGDH antibody 24813-1-AP | Download protocol |
| IF protocol for DMGDH antibody 24813-1-AP | Download protocol |
| IHC protocol for DMGDH antibody 24813-1-AP | Download protocol |
| WB protocol for DMGDH antibody 24813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DMGDH Polyclonal antibody (Cat# 24813-1-AP) has 3 citations published in Nature Communications, Oncotarget, and Journal of Proteome Research. The cited studies span neuroscience (cortical malformation in mice), hepatocellular carcinoma metastasis, and urea cycle proteomics in liver cancer, with applications including IF, WB, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. | ||
Oncotarget Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo. | ||
J Proteome Res Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion. |
Reviews
What customers say
The single review for this antibody is highly positive, with a 5-star rating. The reviewer used it for Western Blot on mouse liver tissue at a 1:4000 dilution and reported a strong signal with clean background, calling it a "great antibody." Overall, the feedback highlights excellent specificity and sensitivity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (08-15-2024) | Great antibody. Strong signal and clean background
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