DNAI2 Polyclonal antibody, PBS Only

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• DNAI2 Polyclonal antibody, PBS Only (17533-1-PBS)

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  WB analysis of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  WB analysis using 17533-1-AP (same clone as 17533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 17533-1-AP (same clone as 17533-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17533-1-AP (same clone as 17533-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 17533-1-AP (same clone as 17533-1-PBS)

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:1000) with mouse testis tissue lysate 1520 ug. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 17533-1-AP (same clone as 17533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 17533-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

CILD9, Dynein axonemal intermediate chain 2

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Product Information

17533-1-PBS targets DNAI2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11585

  Product name: Recombinant human DNAI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 239-593 aa of BC039582

  Sequence: GQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFTELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA

• Full Name: dynein, axonemal, intermediate chain 2
• Calculated Molecular Weight: 593 aa, 67 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC039582
• Gene Symbol: DNAI2
• Gene ID (NCBI): 64446
• RRID: AB_2096670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZS0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.