DNAJB14 Polyclonal antibody, PBS Only

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• DNAJB14 Polyclonal antibody, PBS Only (16501-1-PBS)

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  WB analysis of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis using 16501-1-AP (same clone as 16501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 16501-1-AP (same clone as 16501-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16501-1-AP (same clone as 16501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

DNAJB14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16501-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EGNR9427, PRO34683, UNQ9427/PRO34683

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Product Information

16501-1-PBS targets DNAJB14 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9668

  Product name: Recombinant human DNAJB14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-254 aa of BC022248

  Sequence: MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLM

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 14
• Calculated Molecular Weight: 379 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022248
• Gene Symbol: DNAJB14
• Gene ID (NCBI): 79982
• RRID: AB_2094414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TBM8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.