CoraLite®594-conjugated DNAJB6 Monoclonal antibody

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  IF Staining of HepG2 using CL594-66587

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 DNAJB6 antibody (CL594-66587, Clone: 1A1D2 ) at dilution of 1:200, CL488-Phalloidin (green).

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  FC experiment of Jurkat using CL594-66587

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNAJB6 (CL594-66587, Clone:1A1D2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

DNAJB6 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-66587

Clone No.1A1D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66587 targets DNAJB6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJB6 fusion protein Ag2258
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 6
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa, 38 kDa
• GenBank Accession Number: BC000177
• Gene Symbol: DNAJB6
• Gene ID (NCBI): 10049
• RRID: AB_3084716
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75190
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.