DRP1 (N-terminal) Polyclonal antibody

DRP1 (N-terminal) Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC,ELISA

Conjugate

Unconjugated

Cat no : 26187-1-AP

Synonyms

DLP1, DNM1L, DRP1



Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26187-1-AP is DRP1 (N-terminal) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRP1 (N-terminal) fusion protein Ag24249
Full Name dynamin 1-like
Calculated molecular weight 710 aa, 79 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC024590
Gene symbol DNM1L
Gene ID (NCBI) 10059
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DRP1 (N-terminal) antibody 26187-1-APDownload protocol
IP protocol for DRP1 (N-terminal) antibody 26187-1-APDownload protocol
FC protocol for DRP1 (N-terminal) antibody 26187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

DUSP1 overexpression attenuates renal tubular mitochondrial dysfunction by restoring Parkin-mediated mitophagy in diabetic nephropathy.

Authors - Chang Lu
humanWB

EMBO Mol Med

High-throughput screening identifies suppressors of mitochondrial fragmentation in OPA1 fibroblasts.

Authors - Emma Cretin