CoraLite® Plus 488-conjugated DRP1 (N-terminal) Polyclonal antibody

DRP1 (N-terminal) Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26187

Synonyms

DLP1, DNM1L, Dnm1p/Vps1p like protein, DRP1, DRP1 (N-terminal), DVLP, DYMPLE, dynamin 1 like, Dynamin 1 like protein, Dynamin like protein, Dynamin like protein 4, Dynamin like protein IV, Dynamin related protein 1, DYNIV 11, FLJ41912, HDYNIV, VPS1

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Validation Data Gallery

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  FC experiment of HeLa using CL488-26187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DRP1 (N-terminal) (CL488-26187) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-26187 is DRP1 (N-terminal) Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRP1 (N-terminal) fusion protein Ag24249
• Full Name: dynamin 1-like
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC024590
• Gene Symbol: DNM1L
• Gene ID (NCBI): 10059
• RRID: AB_2919207
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C