Tested Applications
| Positive WB detected in | TDP-43-flag Transfected HEK-293T cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293T cells |
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80801-2-RR targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, IP-MS, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2329 Product name: Recombinant DYKDDDDK tag protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: DYKDDDDK Predict reactive species |
| Full Name | DYKDDDDK tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3670488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | FLAGTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
| IF protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
| IP protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
| WB protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DYKDDDDK tag Recombinant monoclonal antibody (Cat# 80801-2-RR) has 58 citations published in journals including Cell Research, Neuron, Science Advances, Cell Reports, PNAS, Cell Death & Differentiation, Brain, Nature Communications, and mBio. Research fields span cancer biology, autoimmunity, ferroptosis, virology, neurology, lipid metabolism, epigenetics, and protein post-translational modifications. Cited applications include WB, IP, CoIP, IF, IHC, and IP-MS, with reactivity in human, mouse, and chicken.
| Species | Application | Title |
|---|---|---|
Cell Res Sensing of DNA double-strand breaks by the NHEJ system stabilizes RORγt transcriptional activity and shapes Th17 pathogenicity in autoimmunity | ||
Redox Biol Gut flora-derived succinate exacerbates Allergic Airway Inflammation by promoting protein succinylation | ||
J Exp Clin Cancer Res SLAMF7 promotes TCRαβ(+) double negative T cell antitumor activity through enhancing glutamine metabolism. | ||
Sci Adv Golgi-localized phosphatidylinositol 4-kinase β mediates Rab11a activation and trafficking to promote ciliogenesis. | ||
Cell Death Differ GIPC1 governed ferroptosis by regulating DECR1-modulating lipid homeostasis during dilated cardiomyopathy (DCM) |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user (1/5000 dilution, HEK 293 lysates) reported excellent, very clean blots with clear FLAG detection in over-expressed samples and no signal in controls. The other user, who frequently uses it for FLAG-tagged constructs, highlighted its reliability, minimal background, and competitive pricing. Overall, reviewers consistently praise its specificity, low background, and value.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lianne (Verified Customer) (07-14-2026) | HEK 293 total cell lysates run on 12% SDS-PAGE gel. Subsequent PVDF western blots probed with anti-FLAG 1:5000. In image, FLAG detected in (B) containing over-expressed FLAG-tagged protein, but not in (A) where there is no FLAG-tagged protein present. Excellent antibody, very clean western blots.
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Jai (Verified Customer) (06-25-2025) | I use this antibody very frequently for WBs as my constructs are usually FLAG tagged. Very reliable and almost no background labelling. Very good price as well compared to competitors
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