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DYNC1H1 Polyclonal antibody, PBS Only (12345-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 12345-1-AP (same clone as 12345-1-PBS)
Various lysates were subjected to Tris-Acetate gel system followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
WB analysis using 12345-1-AP (same clone as 12345-1-PBS)
Various lysates were subjected to Tris-Acetate gel system followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 12345-1-AP (same clone as 12345-1-PBS)
IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DYNC1H1 Polyclonal antibody, PBS Only

Name: DYNC1H1 Polyclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 12345-1-PBS-100UG

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12345-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DYNC1H 1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12345-1-PBS targets DYNC1H1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2999 Product name: Recombinant human KIAA0325 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 590-989 aa of BC021297 Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE Predict reactive species
Full Name	dynein, cytoplasmic 1, heavy chain 1
Calculated Molecular Weight	4646 aa, 532 kDa
Observed Molecular Weight	532 kDa
GenBank Accession Number	BC021297
Gene Symbol	DYNC1H1
Gene ID (NCBI)	1778
RRID	AB_2261765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14204
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.