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DZIP1 Polyclonal antibody, PBS Only (13779-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 13779-1-AP (same clone as 13779-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 13779-1-AP (same clone as 13779-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 13779-1-AP (same clone as 13779-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 13779-1-AP (same clone as 13779-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 13779-1-AP (same clone as 13779-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 13779-1-AP (same clone as 13779-1-PBS)
IP result of anti-DZIP1 (IP:13779-1-AP, 4ug; Detection:13779-1-AP 1:300) with HEK-293 cells lysate 880ug. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 13779-1-AP (same clone as 13779-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 13779-1-AP (same clone as 13779-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 13779-1-AP (same clone as 13779-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13779-1-AP (DZIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DZIP1 Polyclonal antibody, PBS Only

Name: DZIP1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13779-1-PBS

DZIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13779-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DZIP, DAZ-interacting zinc finger protein 1, DAZ-interacting protein 1/2, DAZ interacting protein 1/2, DAZ interacting protein 1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13779-1-PBS targets DZIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4772 Product name: Recombinant human DZIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 496-867 aa of BC041804 Sequence: FSSHILEPIEELSEEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGISSDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDTLSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSSEEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAGVAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLEEEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLVTVTDWSDTSDV Predict reactive species
Full Name	DAZ interacting protein 1
Calculated Molecular Weight	867 aa, 98 kDa
Observed Molecular Weight	100-113 kDa
GenBank Accession Number	BC041804
Gene Symbol	DZIP1
Gene ID (NCBI)	22873
RRID	AB_2096927
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86YF9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126).