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  • KD/KO Validated

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20874-1-AP

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin



Tested Applications

Positive WB detected inA431 cells, DU 145 cells, mouse testis tissue, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, mouse colon tissue, mouse skin tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, mouse small intestine tissue, human breast cancer tissue, mouse embryo tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20874-1-AP is E-cadherin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, canine, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 120-125 kDa
GenBank accession numberBC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
FC protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

Authors - Yanpu Chen
humanWB

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li
mouseWB

Hepatology

The Mutational Features of Aristolochic Acid-Induced Mouse and Human Liver Cancers.

Authors - Zhao-Ning Lu
mouseIHC

ACS Nano

Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.

Authors - Yakun Wu
mouseIF

Nat Commun

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.

Authors - Hans I-Chen Harn
mouseIHC

Nat Commun

Mammary-specific expression of Trim24 establishes a mouse model of human metaplastic breast cancer.

Authors - Vrutant V Shah

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Davide (Verified Customer) (11-22-2022)

The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)
FH

Sophie (Verified Customer) (02-07-2022)

Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Martin (Verified Customer) (12-13-2017)

  • Applications: Western Blot, Immunofluorescence