- Featured Product
- KD/KO Validated
E-cadherin Polyclonal antibody
E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20874-1-AP
Validation Data Gallery
|Positive WB detected in||T-47D cells, DU 145 cells, A431 cells, mouse testis tissue, MCF-7 cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, mouse colon tissue, human hepatocirrhosis tissue, mouse liver tissue, human colon tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells, human breast cancer tissue, mouse embryo tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:4000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20874-1-AP is E-cadherin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, cow, human, mouse, pig, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||E-cadherin fusion protein Ag14973|
|Full Name||cadherin 1, type 1, E-cadherin (epithelial)|
|Calculated molecular weight||882 aa, 97 kDa|
|Observed molecular weight||125 kDa|
|GenBank accession number||BC141838|
|Gene ID (NCBI)||999|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-kDa intact E-cadherin and its 80-kDa trypsin-cleaved fragment.
|Product Specific Protocols|
|WB protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IHC protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IF protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IP protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|FC protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Aging (Albany NY)
A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.
Keratinocytes Isolated From Individual Cleft Lip/Palate Patients Display Variations in Their Differentiation Potential in vitro.
Med Sci Monit
miR-200c/141 Regulates Breast Cancer Stem Cell Heterogeneity via Targeting HIPK1/β-Catenin Axis.
Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.
Mol Med Rep
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (01-30-2019)
This antibody working very good for human cancer cells in western blotting (1:1000)
Martin (Verified Customer) (12-13-2017)