CoraLite®594 Anti-Mouse E-cadherin Rabbit Recombinant Antibody

E-cadherin Recombinant Antibody for FC

Cat No. CL594-98140
Clone No.241282C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse, canine

Applications

FC

241282C1, cadherin 1, Cdh1, Ecad, L CAM

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inMDCK cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98140 targets E-cadherin in FC applications and shows reactivity with mouse, canine samples.

Tested Reactivity mouse, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen E-cadherin fusion protein Eg0975 Predict reactive species
Full Name cadherin 1
Calculated Molecular Weight98kDa
GenBank Accession NumberNM_009864.3
Gene Symbol E-cadherin
Gene ID (NCBI) 12550
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09803
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
FC protocol for CL594 E-cadherin antibody CL594-98140Download protocol
Standard Protocols
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