CoraLite® Plus 647 Anti-Mouse E-cadherin Rabbit Recombinant Antibody

E-cadherin Recombinant Antibody for FC

Cat No. CL647-98140
Clone No.241282C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse, canine

Applications

FC

241282C1, cadherin 1, Cdh1, Ecad, L CAM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inMDCK cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98140 targets E-cadherin in FC applications and shows reactivity with mouse, canine samples.

Tested Reactivity mouse, canine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen E-cadherin fusion protein Eg0975 Predict reactive species
Full Name cadherin 1
Calculated Molecular Weight98kDa
GenBank Accession NumberNM_009864.3
Gene Symbol E-cadherin
Gene ID (NCBI) 12550
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09803
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 E-cadherin antibody CL647-98140Download protocol
Standard Protocols
Click here to view our Standard Protocols
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