CoraLite®647-conjugated EED Monoclonal antibody

EED Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

1D11D4

Cat no : CL647-67756

Synonyms

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1



Tested Applications

Positive IF detected inmouse testis tissue
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67756-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

CL647-67756 targets EED in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EED fusion protein Ag10369
Full Name embryonic ectoderm development
Calculated molecular weight 466 aa, 53 kDa
GenBank accession numberBC068995
Gene symbol EED
Gene ID (NCBI) 8726
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
IF protocol for CL647 EED antibody CL647-67756Download protocol
FC protocol for CL647 EED antibody CL647-67756Download protocol
Standard Protocols
Click here to view our Standard Protocols