CoraLite®594-conjugated EEF1D Monoclonal antibody

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  IF Staining of HeLa using CL594-60085

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60085 (EEF1D antibody) at dilution of 1:100.

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  IF Staining of HeLa using CL594-60085

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 EEF1D antibody (CL594-60085, Clone: 3B1B11 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL594-60085

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEF1D (CL594-60085, Clone:3B1B11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

EEF1D Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-60085

Clone No.3B1B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60085 targets EEF1D in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0983

  Product name: Recombinant human EEF1D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 347-647 aa of BC007847

  Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

• Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC007847
• Gene Symbol: EEF1D
• Gene ID (NCBI): 1936
• RRID: AB_2883420
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P29692
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )