EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G7C6

Cat no : 68148-1-Ig

Synonyms

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

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Validation Data Gallery

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  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IF Staining of HeLa using 68148-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 68148-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68148-1-Ig targets EEF1G in WB, IF, ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

• Tested Reactivity: Human, Pig, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF1G fusion protein Ag0218
• Full Name: eukaryotic translation elongation factor 1 gamma
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC006520
• Gene Symbol: EEF1G
• Gene ID (NCBI): 1937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.