EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E4D5

Cat no : 67550-1-Ig

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67550-1-Ig targets EEF2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EEF2 fusion protein Ag13859
Full Name eukaryotic translation elongation factor 2
Calculated molecular weight 858 aa, 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC126259
Gene symbol EEF2
Gene ID (NCBI) 1938
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EEF2 antibody 67550-1-IgDownload protocol
IHC protocol for EEF2 antibody 67550-1-IgDownload protocol
IF protocol for EEF2 antibody 67550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols