CoraLite®555-conjugated EEF2 Monoclonal antibody

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  IF Staining of HepG2 using CL555-67550

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 EEF2 antibody (CL555-67550, Clone: 1E4D5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-67550

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human EEF2 (CL555-67550, Clone:1E4D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

EEF2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL555-67550

Clone No.1E4D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

1E4D5, EC:3.6.5.-, EF 2, EF2, EF-2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67550 targets EEF2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13859

  Product name: Recombinant human EEF2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 509-858 aa of BC126259

  Sequence: VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• RRID: AB_2919703
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Excitation Laser: Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13639
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.