CoraLite®594-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E4D5

Cat no : CL594-67550

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67550

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EEF2 antibody (CL594-67550, Clone: 1E4D5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67550

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human EEF2 (CL594-67550, Clone:1E4D5) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL594-67550 targets EEF2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF2 fusion protein Ag13859
• Full Name: eukaryotic translation elongation factor 2
• Calculated molecular weight: 858 aa, 95 kDa
• GenBank accession number: BC126259
• Gene symbol: EEF2
• Gene ID (NCBI): 1938
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C