CoraLite®555-conjugated EIF2A/CDA02 Monoclonal antibody

• 

  IF Staining of HepG2 using CL555-66482

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 EIF2A/CDA02 antibody (CL555-66482, Clone: 1G7H4 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

EIF2A/CDA02 Monoclonal Antibody for IF/ICC

Cat No. CL555-66482

Clone No.1G7H4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

CDA02, EIF 2A, EIF2A, MST089, MSTP004, MSTP089

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66482 targets EIF2A/CDA02 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22041

  Product name: Recombinant human EIF2A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 238-585 aa of BC011885

  Sequence: IASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGIFPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQLEKNQLEKIQKETALLQELEDLKLGI

• Full Name: eukaryotic translation initiation factor 2A, 65kDa
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011885
• Gene Symbol: EIF2A
• Gene ID (NCBI): 83939
• RRID: AB_2934674
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BY44
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.