EIF4G3 Polyclonal antibody, PBS Only

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• EIF4G3 Polyclonal antibody, PBS Only (11281-1-PBS)

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  WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11281-1-AP (same clone as 11281-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

  IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11281-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

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Product Information

11281-1-PBS targets EIF4G3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1811

  Product name: Recombinant human EIF4G3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 197-515 aa of BC030578

  Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC

• Full Name: eukaryotic translation initiation factor 4 gamma, 3
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 70 kDa, 250 kDa
• GenBank Accession Number: BC030578
• Gene Symbol: EIF4G3
• Gene ID (NCBI): 8672
• RRID: AB_2877754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43432
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.