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Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR)

Anti-Human EMR2/CD312 Rabbit Recombinant Antibody

EMR2/CD312 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98260-1-RR

Clone No. 242042B11

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

EMR2, 242042B11, ADGRE2, Adhesion G protein-coupled receptor E2, CD312

Formulation: PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of human PBMCs using 98260-1-RR
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98260-1-RR
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human EMR2/CD312 Rabbit Recombinant Antibody (98260-1-RR, Clone: 242042B11)(red) or 0.125 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98260-1-RR
Biolayer interferometry (BLl) kinetic assays of 98260-1-RR against Human EMR2/CD312 were performed. The affinity constant is 0.529 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for EMR2/CD312 in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.13 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98260-1-RR targets EMR2/CD312 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2479 Product name: Recombinant Human EMR2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-260 aa of NM_013447.3 Sequence: QDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINECATLSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCLPGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCE Predict reactive species
Full Name	egf-like module containing, mucin-like, hormone receptor-like 2
Calculated Molecular Weight	90 kDa
GenBank Accession Number	NM_013447.3
Gene Symbol	EMR2
Gene ID (NCBI)	30817
RRID	AB_3745926
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UHX3-1
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as CD312 or ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).