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EPHB4 Polyclonal antibody, PBS Only (20883-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 20883-1-AP (same clone as 20883-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20883-1-AP (same clone as 20883-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
WB analysis of HT-1080 using 20883-1-AP (same clone as 20883-1-PBS)
HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
WB analysis of DU 145 using 20883-1-AP (same clone as 20883-1-PBS)
DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 20883-1-AP (same clone as 20883-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.
IF Staining of HT-29 using 20883-1-AP (same clone as 20883-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

EPHB4 Polyclonal antibody, PBS Only

Name: EPHB4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20883-1-PBS

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20883-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

20883-1-PBS targets EPHB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10033 Product name: Recombinant human EPHB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 639-987 aa of BC052804 Sequence: KKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY Predict reactive species
Full Name	EPH receptor B4
Calculated Molecular Weight	987 aa, 108 kDa
Observed Molecular Weight	110-120 kDa
GenBank Accession Number	BC052804
Gene Symbol	EPHB4
Gene ID (NCBI)	2050
RRID	AB_10897158
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54760
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker.