EPO Monoclonal antibody

EPO Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11F3

Cat no : 66975-1-Ig

Synonyms

EP, EPO, Epoetin, erythropoietin, MVCD2



Tested Applications

Positive WB detected inrecombinant human EPO (cat.NO. HZ-1168)
Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66975-1-Ig targets EPO in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPO fusion protein Ag23054
Full Name erythropoietin
Calculated molecular weight 193 aa, 21 kDa
GenBank accession numberBC093628
Gene symbol EPO
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Protocols

Product Specific Protocols
WB protocol for EPO antibody 66975-1-IgDownload protocol
IHC protocol for EPO antibody 66975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols