EPO Monoclonal antibody

EPO Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, mouse and More (2)







Cat no : 66975-1-Ig


EP, EPO, Epoetin, erythropoietin, MVCD2

Tested Applications

Positive WB detected inrecombinant human EPO (cat.NO. HZ-1168)
Positive IHC detected inhuman liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

66975-1-Ig targets EPO in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPO fusion protein Ag23054
Full Name erythropoietin
Calculated Molecular Weight 193 aa, 21 kDa
GenBank Accession NumberBC093628
Gene Symbol EPO
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.


Product Specific Protocols
WB protocol for EPO antibody 66975-1-IgDownload protocol
IHC protocol for EPO antibody 66975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Oncol

TCF11 Has a Potent Tumor-Repressing Effect Than Its Prototypic Nrf1α by Definition of Both Similar Yet Different Regulatory Profiles, With a Striking Disparity From Nrf2.

Authors - Meng Wang

Front Cardiovasc Med

Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α Inhibition.

Authors - Hari Prasad Osuru

Am J Physiol Renal Physiol

The hypoxia-inducible factor-α prolyl hydroxylase inhibitor FG4592 ameliorates renal fibrosis by inducing the H3K9 demethylase JMJD1A

Authors - Takeshi Ike


Perfluorocarbon Nanoparticles Incorporating GB: Artificial O2 Carrier with Antioxidant Activity and Antithrombotic Effect

Authors - Wei Meng