ERBB4 Polyclonal antibody, PBS Only

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• ERBB4 Polyclonal antibody, PBS Only (19943-1-PBS)

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  WB analysis of mouse brain using 19943-1-AP (same clone as 19943-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

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  WB analysis using 19943-1-AP (same clone as 19943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 19943-1-AP (same clone as 19943-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 19943-1-AP (same clone as 19943-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 19943-1-AP (same clone as 19943-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 19943-1-AP (same clone as 19943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 19943-1-AP (same clone as 19943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 19943-1-AP (same clone as 19943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 19943-1-AP (same clone as 19943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 19943-1-AP (same clone as 19943-1-PBS)

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (green) and ErbB4 (red, Cat. No 19943-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 488 secondary antibody. ErbB4 is a key member of ErbB signaling and involved in development and was visualized with an Alexa Fluor 555 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 19943-1-PBS in a different storage buffer formulation.

ERBB4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19943-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

4ICD, E4ICD, EC:2.7.10.1, ERBB4 intracellular domain, HER4

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Product Information

19943-1-PBS targets ERBB4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• Calculated Molecular Weight: 147 kDa
• Observed Molecular Weight: 180 kDa, 147 kDa
• GenBank Accession Number: NM_001042599
• Gene Symbol: ERBB4
• Gene ID (NCBI): 2066
• RRID: AB_10646486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15303
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).