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ERCC2 Polyclonal antibody, PBS Only (10818-1-PBS)

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WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)
WB result of ERCC2 antibody (10818-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 10818-1-AP (same clone as 10818-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)
IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

ERCC2 Polyclonal antibody, PBS Only

Name: ERCC2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10818-1-PBS

ERCC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10818-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BTF2 p80, COFS2, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10818-1-PBS targets ERCC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1038 Product name: Recombinant human ERCC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC008346 Sequence: MRELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGIYNLDDLKALGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYRRLVEGLREASAARETDAHLANPVLPDEVLQEAVPGSIRT Predict reactive species
Full Name	excision repair cross-complementing rodent repair deficiency, complementation group 2
Calculated Molecular Weight	87 kDa
Observed Molecular Weight	80 kDa
GenBank Accession Number	BC008346
Gene Symbol	ERCC2
Gene ID (NCBI)	2068
RRID	AB_2231330
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P18074
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.