ERp72 Polyclonal antibody, PBS Only

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• ERp72 Polyclonal antibody, PBS Only (14712-1-PBS)

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  WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 14712-1-AP (same clone as 14712-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14712-1-AP (same clone as 14712-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

  IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 14712-1-AP (same clone as 14712-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 14712-1-AP (same clone as 14712-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 14712-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

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Product Information

14712-1-PBS targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6431

  Product name: Recombinant human ERp72 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 294-645 aa of BC000425

  Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

• Full Name: protein disulfide isomerase family A, member 4
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC000425
• Gene Symbol: ERp72
• Gene ID (NCBI): 9601
• RRID: AB_2160973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13667
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.