|Positive WB detected in||mouse brain tissue, L02 cells, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14712-1-AP targets ERp72 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ERp72 fusion protein Ag6431|
|Full Name||protein disulfide isomerase family A, member 4|
|Calculated molecular weight||73 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||BC000425|
|Gene ID (NCBI)||9601|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.
|Product Specific Protocols|
|WB protocol for ERp72 antibody 14712-1-AP||Download protocol|
|IHC protocol for ERp72 antibody 14712-1-AP||Download protocol|
|IF protocol for ERp72 antibody 14712-1-AP||Download protocol|
|IP protocol for ERp72 antibody 14712-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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