ERp72 Antibody

ERp72 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14712-1-AP

Synonyms

ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4



Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14712-1-AP targets ERp72 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6431
Full Name protein disulfide isomerase family A, member 4
Calculated molecular weight 73 kDa
Observed molecular weight 72 kDa
GenBank accession number BC000425
Gene symbol PDIA4
Gene ID (NCBI) 9601
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Protocols

Product Specific Protocols
WB protocol for ERp72 antibody 14712-1-APDownload protocol
IHC protocol for ERp72 antibody 14712-1-APDownload protocol
IF protocol for ERp72 antibody 14712-1-APDownload protocol
IP protocol for ERp72 antibody 14712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Asian Pac J Cancer Prev

Inhibition by Imatinib of Expression of O-glycan-related Glycosyltransferases and Tumor-associated Carbohydrate Antigens in the K562 Human Leukemia Cell Line.

Authors - Sun Qi-Chang QC
humanIF

Biochem Biophys Res Commun

Identification and characterization of an endoplasmic reticulum localization motif.

Authors - Yong-Wei Nie
humanWB

Exp Eye Res

Mapping wild-type and R345W fibulin-3 intracellular interactomes.

Authors - John D Hulleman
humanIF

Biochem J

N-glycosylation is required for FDNC5 stabilization and irisin secretion.

Authors - Yong Wei Nie
monkeyIF

J Biol Chem

Receptor-like protein-tyrosine phosphatase α enhances cell surface expression of neural adhesion molecule NB-3.

Authors - Ye Haihong H
humanWB,IHC

Oncotarget

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

Authors - Soma Samanta