ESM1 Recombinant antibody

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  WB analysis using 86027-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86027-1-RR (ESM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ESM1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86027-1-RR

Clone No.250287H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1

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Tested Applications

• Positive WB detected in: HUVEC cells, PC-3 cells, DU 145 cells, A2780 cells, SKOV-3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86027-1-RR targets ESM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ESM1 fusion protein Ag3019
• Full Name: endothelial cell-specific molecule 1
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC011989
• Gene Symbol: ESM1
• Gene ID (NCBI): 11082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ30
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESM1, also known as endocan or endothelial cell-specific molecule 1, is a protein-coding gene that encodes a secreted proteoglycan primarily expressed in endothelial cells of human tissues, including lung, kidney, and vascular systems. The protein consists of a 20 kDa core protein with a dermatan sulfate chain, forming a 50 kDa mature proteoglycan. ESM1 plays a crucial role in endothelial cell proliferation, migration, and angiogenesis (blood vessel formation). It modulates vascular development and maintains endothelial homeostasis. Overexpression of ESM1 is observed in cancers such as gastric, cervical, and lung tumors, where it promotes angiogenesis and tumor progression via pathways like VEGF/ERK signaling. Elevated serum ESM1 levels are associated with poor clinical outcomes in cancer patients.