EXD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20138-1-AP

Synonyms

C14orf114, EXD2, EXDL2

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Validation Data Gallery

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  WB analysis of mouse lung using 20138-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 20138-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 20138-1-AP

  IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.

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  IHC staining of human testis using 20138-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20138-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20138-1-AP

  Immunofluorescent analysis of HepG2 cells, using EXD2 antibody 20138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse brain tissue, mouse pancreas tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20138-1-AP is EXD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXD2 fusion protein Ag14090
• Full Name: exonuclease 3'-5' domain containing 2
• Calculated Molecular Weight: 70 kD aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC001962
• Gene Symbol: EXD2
• Gene ID (NCBI): 55218
• RRID: AB_10665419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.; Authors - Xikui Sun ; KO Validated
mouse	WB,IF	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu