Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-20138 targets EXD2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14090 Product name: Recombinant human EXD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 147-496 aa of BC001962 Sequence: DDHSSWRKVLEKCQGVVDIPFRSKGMSRLGEEVNGEATESQQKPRNKKSKMDGMVPGNHQGRDPRKHKRKPLGVGYSARKSPLYDNCFLHAPDGQPLCTCDRRKAQWYLDKGIGELVSEEPFVVKLRFEPAGRPESPGDYYLMVKENLCVVCGKRDSYIRKNVIPHEYRKHFPIEMKDHNSHDVLLLCTSCHAISNYYDNHLKQQLAKEFQAPIGSEEGLRLLEDPERRQVRSGARALLNAESLPTQRKEELLQALREFYNTDVVTEEMLQEAASLETRISNENYVPHGLKVVQCHSQGGLRSLMQLESRWRQHFLDSMQPKHLPQQWSVDHNHQKLLRKFGEDLPIQLS Predict reactive species |
| Full Name | exonuclease 3'-5' domain containing 2 |
| Calculated Molecular Weight | 70 kD aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC001962 |
| Gene Symbol | EXD2 |
| Gene ID (NCBI) | 55218 |
| RRID | AB_3672713 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVH0 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXD2 (Exonuclease 3'-5' Domain Containing 2), is a protein with exonuclease activity of 3'-5' nucleic acid, which is involved in many biological processes in cells, including DNA repair, RNA processing and mitochondrial translation. EXD2 protein is mainly located in mitochondrial outer membrane, intermediate fiber cytoskeleton and DNA damage site. This protein may be a potential drug target in some cancers. For example, in ALT (telomere selective elongation) dependent cancer, the co-elimination of EXD2 with BLM, DNA2 or POLD3 may mediate synthetic lethality. In addition, EXD2 inhibitors may exert anticancer effects by inhibiting mitochondrial translation and metabolic reprogramming. It has two isoforms with molecular weights of 56 kDa and 70 kDa respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EXD2 antibody CL488-20138 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
