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Endothelin 3 Polyclonal antibody, PBS Only (27871-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 27871-1-AP (same clone as 27871-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 27871-1-AP (Endothelin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 27871-1-AP (same clone as 27871-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.
IF Staining of BxPC-3 using 27871-1-AP (same clone as 27871-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

Endothelin 3 Polyclonal antibody, PBS Only

Name: Endothelin 3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27871-1-PBS

Endothelin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 27871-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EDN3, Endothelin-3, ET 3, ET3, ET-3

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27871-1-PBS targets Endothelin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27393 Product name: Recombinant human EDN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-198 aa of BC053866 Sequence: SQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVE Predict reactive species
Full Name	endothelin 3
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC053866
Gene Symbol	Endothelin 3
Gene ID (NCBI)	1908
RRID	AB_3086000
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14138
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).